Chemisosmosis
In chemiosmosis, chloroplasts, protons (H+) are pumped
through electron transfer to one side of the membrane, creating a gradient,
which then allows them to diffuse back across through ATP synthase. This
process allows ATP to be produced from ADP + P.
In chloroplast, the H+ are pumped into the thylakoid membrane space and then diffuse back out to the stroma. This causes ATP (like NADPH) to be produced in the stroma, which makes it readily available for the Calvin cycle.
An increase in light causes an increase in the amount of H+
that is pumped into the thylakoid membrane. The pH
gradient will not occur if there is no light, as the process needs light energy
to work.
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